Corresponding Author

Jian-ying QU(lilyqujy@yahoo.com.cn)


A novel laccase (Lac) biosensor was fabricated successfully by means of entrapping Lac into bimetallic Pt/Au particles and chitosan/silica (CS-SiO2) sol-gel hybrid films, and then applied to determine catechol (CC) concentration. The experiment results showed that the biosensor can electrocatalyze the reduction of CC, and the peak current increased linearly with the concentration of CC in the range of 8×10-7 ~ 1×10-4 mol·L-1 (r = 0.9993) with a detection limit of 7.9×10-8 mol·L-1 by cyclic voltammetric response. The biosensor exhibited good sensitivity, stability and reproducibility, with a broad linear range for potential applications.

Graphical Abstract

Publication Date


Online Available Date


Revised Date


Received Date



[1] Zhang Y(章毅), Zeng G M(曾光明), Tang L(汤琳), et al. Catechol biosensor based on immobilizing laccase to modified core-shell magnetic nanoparticles supported on carbon paste electrode[J]. Environment Science(环境科学), 2007, 28(10): 2320-2325.
[2] Liu Y, Qu X H, Guo H W, et al. Facile preparation of amperometric laccase biosensor with multifunction based on the matrix of carbon nanotubes-chitosan composite[J]. Biosensors and Bioelectronics, 2006, 21(12): 2195-2201.
[3] Li Y Y(李玉英), Yang Z Y(杨震宇). Research progress nature and application of laccase[J]. Jiangxi Science(江西科学), 2009, 27(5): 680-684.
[4] Hu Q F(胡秋芬), Yang G Y(杨光宇), Huang Z J(黄章杰), et al. Determination of phenols by solid phase extraction and high performance liquid chromatography[J]. Chinese Journal of Analytical Chemistry(分析化学), 2002, 30(5): 560-563.
[5] Bagheri H, Saber A, Mousavi S R. Immersed solvent microextraction of phenol and chlorophenols from water samples followed by gas chromatography-mass spectrometry[J]. Journal of Chromatography A, 2004, 1046(1/2): 27-33.
[6] Chen H(陈慧), Wang J C(王九春), Zhou M(周敏), et al. Determination of pyrocatechol in waters by means of flow-injection chemiluminescence[J]. Journal of Lanzhou University of Technology(兰州理工大学学报), 2010, 36(2): 52-55.
[7] Tan X C(谭学才), Liang R P(梁汝萍), Li M(李荫), et al. An amperometric hydrogen peroxide biosensor based on chitosan/silica sol-gel composite films incorporating horseradish peroxidase[J]. Chinese Journal of Analysis Laboratory(分析试验室), 2004, 23(6): 1-4.
[8] Xu J Z(徐娇珍), Yang P(杨平), Hua N P(华南平), et al. Preparation and characterization of bimetallic Pt/Au nanoparticles[J]. Chemical Research and Application(化学研究与应用), 2003, 15(6): 838-840.
[9] Tang Z X(唐振兴), Shi L E(石陆娥), Qian J Q(钱俊青). Study of adsorption mechanism of chitosan gel for protein[J]. Fine Chemicals(精细化工), 2004, 21(11): 833-836.
[10] Klotzbach T, Watt M, Ansari Y, et al. Effects of hydrophobic modification of chitosan and nafion on transport properties, ion-exchange capacities, and enzyme immobilization[J]. Journal of Membrane Science, 2006, 282(1/2): 276-283.
[11] Li W J, Yuan R, Chai Y Q, et al. Immobilization of horseradish peroxidase on chitosan/silica sol-gel hybrid membranes for the preparation of hydrogen peroxide biosensor[J]. Journal of Biochemical and Biophysical Methods, 2008, 70(6): 830-837.
[12] Lu H X(卢海霞), Wu Z S(吴再生), Jin X Y(晋晓勇), et al. Novel electrochemical sensor based on seminetwork-type Au nanoparticles labled horseradish peroxidase[J]. Chemical Journal of Chinese Universities(高等学校化学学报), 2009, 30(2): 263-267.
[13] Zhang Y(张雁), Kang T F(康天放), Lu L P(鲁理平), et al. Self-assembled gold nanoparticles modified dlectrode for dlectrochemical detection nitrite[J]. Environmental Science(环境科学), 2011, 32(4): 1127-1132.
[14] Zhang Y(张雁), Kang T F(康天放), Lu L P(鲁理平), et al. Electrochemical determination of nitrite using a gold nanoparticles/(3-aminopropyl)-trimethoxysilane modified sensor[J]. Journal of Analytical Science(分析科学学报), 2009, 25(3): 253-256.
[15] Zhou Y Y(周耀渝), Tang L(汤琳), Li Z(李贞), et al. Preparation of OMC-Au/L-lysine/Au modified glassy carbon electrode and the study on its detection response to hydroquinone and catechol[J]. Environmental Science(环境科学), 2013, 34(3): 1211-1217.
[16] Jiang L Y(姜利英), Cai X X(蔡新霞), Liu H M(刘红敏), et al. A novel glucose biosensor based on thin-film gold electrode modified nanoparticles[J]. Chinese Journal of Analytical Chemistry(分析化学研究简报),2008, 36(11): 1563-1566.
[17] Sun S J(孙双姣), Jiang Z L(蒋治良). Preparation and characterization of gold nanoparticles and their application in biochemical analysis[J]. Precious Metals(贵金属), 2005, 26(30): 55-65.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.