Electrochemistry of Glucose Oxidase Modified on Porin-Phospholipid Biomimic Membrane

Authors

Kun-qi WANG, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;Changchun Institute of Technology, Changchun 1300122, China;
Zuo-ming ZHANG, Jilin University, Changchun 130012, China;Follow
Wei XING, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;Follow
Chang-peng LIU, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
Xing ZHOU, Changchun Institute of Technology, Changchun 1300122, China;

Corresponding Author

Zuo-ming ZHANG(xingwei@ciac.ac.cn);
Wei XING(zmzhang@jlu.edu.cn)

Abstract

Biomimic membrane with nano-channels, which is made up of porin MspA and 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) is constructed on glassy carbon substrate, and glucose oxidase (GOD) is modified on it. The direct electrochemical reaction and electrocatalytic behavior to oxygen and glucose of GOD on the GOD/MspA-DMPC/GC electreode are expounded by the cyclic voltammetric method. The study shows that GOD immobilized on porin MspA and DMPC biomimic membrane displays direct and surface-controlled electrochemical reaction nearby formal potential (E0′) of the flavoprotein active centre (FAD/FADH), and the electrochemical reaction contains two electrons and two protons exchange in 0.1 mmol?L^-1 phosphate buffer solution (PBS) (pH 7.0). Furthermore, it is also discovered that, GOD immobilized on porin MspA and DMPC biomimic membrane possesses an excellent bioelectrocatalytic activity for the reduction of O₂ and the oxidation of glucose. That is to say, the biomimic nano-channels membrane formed by porin MspA and DMPC provides an ideal living environment for GOD. So, the GOD/MspA-DMPC/GC electrode can be utilized in biosensor and biofuel cell in the future.

Graphical Abstract

Keywords

porin MspA, biomimic membrane, glucose oxidase, electrochemistry, 1, 2-dimyristoyl-sn-glycero-3-phosphocholine

DOI Link

https://doi.org/10.13208/j.electrochem.131176

Publication Date

2014-10-28

Online Available Date

2014-04-14

Revised Date

2014-04-09

Received Date

2014-01-22

Recommended Citation

Kun-qi WANG, Zuo-ming ZHANG, Wei XING, Chang-peng LIU, Xing ZHOU. Electrochemistry of Glucose Oxidase Modified on Porin-Phospholipid Biomimic Membrane[J]. Journal of Electrochemistry, 2014 , 20(5): 439-443.
DOI: 10.13208/j.electrochem.131176
Available at: https://jelectrochem.xmu.edu.cn/journal/vol20/iss5/6

References

[1] Perera A S, Wang H W, Shrestha T B, et al. Nanoscopic surfactant behavior of the porin MspA in aqueous media[J]. Beilstein Journal of Nanotechnology, 2013, 4: 278-284.
[2] Engelhardt H, Heinz C, Niederweis M, A Tetrameric porin limits the cell wall permeability of mycobacterium smegmatis[J]. Journal of Biological Chemistry, 2002, 277(40): 37567-37572.
[3] Basel M T, Dani R K, Kang M, et al. Direct observation of gold nanoparticle assemblies with the porin MspA on mica[J]. ACS Nano, 2009, 3(2): 462-466.
[4] Worner M, Niebler S, Gogritchiani E, et al. Nanoarray-surfaces by reconstitution of the porin MspA into stabilized long-chain-lipid-monolayers at a gold-surface[J]. Electroanalysis, 2006, 18(19/20): 1859-1870.
[5] Perera A S, Subbaiyan N K, Kalita M, et al. A hybrid soft solar cell based on the mycobacterial porin MspA linked to a sensitizer-viologen diad[J]. Journal of the American Chemical Society, 2013, 135(18): 6842-6845.
[6] Bossmann S H, Janik K, Pokhrel M R, et al. Reconstitution of a porin from mycobacterium smegmatis at HOPG covered with hydrophobic host layers[J]. Surface and Interface Analysis, 2004, 36(2): 127-134.
[7] Zhang Z L(张治磊), Wang Z N(王志宁), Gao X L(高学理), et al. Progress in supported phospholipid bilayers[J]. Progress in Chemistry(化学进展), 2012, 24(5): 852-861.
[8] Tong Y H(佟月红). Electrochemistry study on mimetic biomembrane[D].Changchun: Changchun Institute of Applied Chemistry Chinese Academy of Sciences, 2001.
[9] Luo L Q(罗立强). Biomembrans and their application in electrochemical biosensor[D].Changchun: Changchun Institute of Applied Chemistry Chinese Academy of Sciences, 2000.
[10] Salamon Z, Tollin G, Cyclic voltammetric behavior of [2Fe-2S] ferredoxins at a lipid bilayer modified electrode[J]. Bioelectrochemistry and Bioenergetics, 1992, 27(3): 381-391.
[11] Gao H(高红), Luo G A(罗国安). Electrochemical properties of bilayer lipid membranes and their applications in biosensors[J]. Journal of Analytical Science(分子科学学报), 2002, 18(1): 70-73.
[12] Li W J(李文娟), Yuan R(袁若), Chai Y Q(柴雅琴). Progress on immobilized techllique of enzyme and nanomaterials-based electrochemicaI enzyme biosensor[J]. Chemical Senors(化学传感器), 2011, 31(2): 1-10.
[13] Wang K, Yang H, Zhu L, et al. Direct electron transfer and electrocatalysis of glucose oxidase immobilized on glassy carbon electrode modified with Nafion and mesoporous carbon FDU-15[J]. Electrochimica Acta, 2009, 54(20): 4626-4630.
[14] Wang K, Yang H, Zhu L, et al. Direct electrochemistry and electrocatalysis of glucose oxidase immobilized on glassy carbon electrode modified by Nafion and ordered mesoporous silica-SBA-15[J]. Journal of Molecular Catalysis B: Enzymatic, 2009, 58(1/4): 194-198.
[15] Wang J Y(王镜岩), Zhu S G(朱圣庚), Xu C F(徐长法). Essential Biochemistry(生物化学教程) [M]. Beijing: Higher Education Press(高等教育出版社), 2008: 348-349.
[16] Liu S, Ju H. Reagentless glucose biosensor based on direct electron transfer of glucose oxidase immobilized on colloidal gold modified carbon paste electrode[J]. Biosensors and Bioelectronics, 2003, 19(3): 177-183.
[17] Hui J, Cui J, Xu G, et al. Direct electrochemistry of glucose oxidase based on nafion-graphene-GOD modified gold electrode and application to glucose detection[J]. Materials Letters, 2013, 108: 88-91.