Electrochemistry of Glucose Oxidase Modified on Porin-Phospholipid Biomimic Membrane

Authors

Kun-qi WANG, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;Changchun Institute of Technology, Changchun 1300122, China;
Zuo-ming ZHANG, Jilin University, Changchun 130012, China;Follow
Wei XING, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;Follow
Chang-peng LIU, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
Xing ZHOU, Changchun Institute of Technology, Changchun 1300122, China;

Corresponding Author

Zuo-ming ZHANG(xingwei@ciac.ac.cn);
Wei XING(zmzhang@jlu.edu.cn)

Abstract

Biomimic membrane with nano-channels, which is made up of porin MspA and 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) is constructed on glassy carbon substrate, and glucose oxidase (GOD) is modified on it. The direct electrochemical reaction and electrocatalytic behavior to oxygen and glucose of GOD on the GOD/MspA-DMPC/GC electreode are expounded by the cyclic voltammetric method. The study shows that GOD immobilized on porin MspA and DMPC biomimic membrane displays direct and surface-controlled electrochemical reaction nearby formal potential (E0′) of the flavoprotein active centre (FAD/FADH), and the electrochemical reaction contains two electrons and two protons exchange in 0.1 mmol?L^-1 phosphate buffer solution (PBS) (pH 7.0). Furthermore, it is also discovered that, GOD immobilized on porin MspA and DMPC biomimic membrane possesses an excellent bioelectrocatalytic activity for the reduction of O₂ and the oxidation of glucose. That is to say, the biomimic nano-channels membrane formed by porin MspA and DMPC provides an ideal living environment for GOD. So, the GOD/MspA-DMPC/GC electrode can be utilized in biosensor and biofuel cell in the future.

Graphical Abstract

Keywords

porin MspA, biomimic membrane, glucose oxidase, electrochemistry, 1, 2-dimyristoyl-sn-glycero-3-phosphocholine

DOI Link

https://doi.org/10.13208/j.electrochem.131176

Publication Date

2014-10-28

Online Available Date

2014-04-14

Revised Date

2014-04-09

Received Date

2014-01-22

Recommended Citation

Kun-qi WANG, Zuo-ming ZHANG, Wei XING, Chang-peng LIU, Xing ZHOU. Electrochemistry of Glucose Oxidase Modified on Porin-Phospholipid Biomimic Membrane[J]. Journal of Electrochemistry, 2014 , 20(5): 439-443.
DOI: 10.13208/j.electrochem.131176
Available at: https://jelectrochem.xmu.edu.cn/journal/vol20/iss5/6