Corresponding Author

Zuo-ming ZHANG(xingwei@ciac.ac.cn);
Wei XING(zmzhang@jlu.edu.cn)


Biomimic membrane with nano-channels, which is made up of porin MspA and 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) is constructed on glassy carbon substrate, and glucose oxidase (GOD) is modified on it. The direct electrochemical reaction and electrocatalytic behavior to oxygen and glucose of GOD on the GOD/MspA-DMPC/GC electreode are expounded by the cyclic voltammetric method. The study shows that GOD immobilized on porin MspA and DMPC biomimic membrane displays direct and surface-controlled electrochemical reaction nearby formal potential (E0′) of the flavoprotein active centre (FAD/FADH), and the electrochemical reaction contains two electrons and two protons exchange in 0.1 mmol?L-1 phosphate buffer solution (PBS) (pH 7.0). Furthermore, it is also discovered that, GOD immobilized on porin MspA and DMPC biomimic membrane possesses an excellent bioelectrocatalytic activity for the reduction of O2 and the oxidation of glucose. That is to say, the biomimic nano-channels membrane formed by porin MspA and DMPC provides an ideal living environment for GOD. So, the GOD/MspA-DMPC/GC electrode can be utilized in biosensor and biofuel cell in the future.

Graphical Abstract


porin MspA, biomimic membrane, glucose oxidase, electrochemistry, 1, 2-dimyristoyl-sn-glycero-3-phosphocholine

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