Preparation of Surface Molecularly Imprinted Magnetic Nanoparticles for Hemoglobin Sensing

Authors

Chun-fang HUANG, Department of Chemistry, Nanchang University, Nanchang 330031, China;School of Chemistry and Chemical Engineering, Jinggangshan University, Ji’an 343009, Jiangxi, China;
Gui-hong YAO, Department of Chemistry, Nanchang University, Nanchang 330031, China;
Jian-ding QIU, Department of Chemistry, Nanchang University, Nanchang 330031, China;Follow

Corresponding Author

Jian-ding QIU(jdqiu@ncu.edu.cn)

Abstract

Surface molecularly imprinted magnetic nanoparticles (NPs) were prepared by employing dopamine (DA) as a functional monomer, hemoglobin (Hb) as a template, H₂PtCl₆ as an oxidant triggered DA polymerization on the surface of the Fe₃O₄ NPs. The Hb and the formed platinum nanoparticles (PtNPs) were embedded into the polydopamine (PDA). After removal of the Hb, the surface molecularly imprinted magnetic nanoparticles (imprinted Fe₃O₄/PDA-PtNPs) were formed, and then simply immobilized on the magnetic glassy carbon electrode (imprinted Fe₃O₄/PDA-PtNPs/MGCE) for Hb sensing. The imprinted Fe₃O₄/PDA-PtNPs were demonstrated magnetic spherical shape with good solubility in water and narrow size distribution. The PtNPs embedded in the imprinted coating could effectively promote the conductivity and the mechanical strength of imprinted cavity. In addition, the imprinted Fe₃O₄/PDA-PtNPs/MGCE could selectively recognize Hb and was used to detect Hb in the range of 0.14 ~ 2.7 μg?mL^-1 with a detection limit of 0.05 μg?mL^-1 (S/N=3).

Graphical Abstract

Keywords

molecularly imprinted polymer, magnetic nanoparticles, dopamine, hemoglobin, electrochemical sensor

DOI Link

https://doi.org/10.13208/j.electrochem.140437

Publication Date

2014-12-28

Online Available Date

2014-07-26

Revised Date

2014-07-21

Received Date

2014-06-03

Recommended Citation

Chun-fang HUANG, Gui-hong YAO, Jian-ding QIU. Preparation of Surface Molecularly Imprinted Magnetic Nanoparticles for Hemoglobin Sensing[J]. Journal of Electrochemistry, 2014 , 20(6): 521-526.
DOI: 10.13208/j.electrochem.140437
Available at: https://jelectrochem.xmu.edu.cn/journal/vol20/iss6/4