Sensitive Detection of Hydroxyl Radical based on Silver-Enhanced Gold Nanoparticle Label

Authors

Yan YANG, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha Hunan 410082, China;College of Chemistry and Pharmaceutical engineering, Nanyang Normal University, Henan Nanyang, 473061, China;
Peng YU, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha Hunan 410082, China;
Xiao-hua ZHANG, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha Hunan 410082, China;Follow
Jin-hua CHEN, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha Hunan 410082, China;Follow

Corresponding Author

Xiao-hua ZHANG(mickyxie@hnu.edu.cn);
Jin-hua CHEN(chenjinhua@hnu.edu.cn)

Abstract

A novel DNA-based electrochemical sensor has been successfully constructed for sensitive detection of hydroxyl radical (·OH) based on the silver-enhanced gold nanoparticle label. Thiolated DNA1 was firstly immobilized on the gold electrode through Au—S bonds. The ·OH generated from Fenton reaction could induce serious oxidative damage of the DNA1 layer on the electrode surface. Then DNA2-functionalized gold nanoparticles (DNA2-AuNPs) were linked on the electrode through the hybridization between DNA2 and undamaged DNA1. Based on the catalytic reduction of silver ion by AuNPs, a silver layer was formed on the surface of AuNPs. The quantitative assay of ·OH was carried out by differential pulse voltammetry (DPV) detection of the deposited silver. Under the optimization conditions, the developed DNA-based biosensor could detect ·OH quantitatively with wide linear range (0.2 ~ 200 μmol·L^-1) and low detection limit (50 nmol·L^-1), and exhibited satisfactory selectivity and reproducibility. This electrochemical biosensor could have potential application in the evaluation of antioxidant capacity.

Graphical Abstract

Keywords

hydroxyl radical, DNA damage, gold nanoparticle, silver-enhancement, electrochemical sensor

DOI Link

https://doi.org/10.13208/j.electrochem.140442

Publication Date

2015-02-28

Online Available Date

2014-09-30

Revised Date

2014-09-25

Received Date

2014-08-04

Recommended Citation

Yan YANG, Peng YU, Xiao-hua ZHANG, Jin-hua CHEN. Sensitive Detection of Hydroxyl Radical based on Silver-Enhanced Gold Nanoparticle Label[J]. Journal of Electrochemistry, 2015 , 21(1): 22-28.
DOI: 10.13208/j.electrochem.140442
Available at: https://jelectrochem.xmu.edu.cn/journal/vol21/iss1/4