Electrochemical Monitoring of Cell Wall-Regulated Transient Extracellular Oxidative Burst from Single Plant Cells

Authors

Jun-tao LIU, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;
Yan-ling LIU, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;
Zhi CHENG, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;
Shi-jing CHEN, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;
Wei-Hua HUANG, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;Follow

Corresponding Author

Wei-Hua HUANG(whhuang@whu.edu.cn)

Abstract

Oxidative burst by rapid, transient generation of reactive oxygen species (ROS) is a fundamental process for plant disease defense and signal transduction. ROS can be protective or toxic depending on their concentrations, and critical balance between ROS production and scavenging is regulated by complex and perfect mechanisms in plant cells. However, the process of rapid and transient ROS burst from plant cells and their regulatory mechanisms are far from being completely understood, owing to the methodological obstacles in real-time monitoring such a fast process. In this work, we showed the application of microelectrode electrochemistry with high spatiotemporal resolution to monitor the kinetics of ROS burst from single plant cell and to investigate the regulatory functions of cell wall. An agarose chip with numerous microwell arrays was fabricated to efficiently trap individual plant cells for electrochemical detection. The results revealed that the oxidative burst from single Arabidopsis thaliana cells is characterized by numerous transient spikes with fast kinetics. Further experiments indicated that intact plant cells released much less H₂O₂ molecules with faster kinetics to the extracellular environment compared with single protoplasts, while the ROS level could be recovered when the activity of cell wall peroxidase (POD) was inhibited. The results provide evidence that cell wall participates in regulating transient oxidative burst transient by a mechanism involving cell wall POD machinery at single cell level. We think that the results presented here would facilitate better understanding on ROS burst and their regulatory mechanisms.

Graphical Abstract

Keywords

oxidative burst, single plant cell, cell wall, microelectrochemical sensor, real-time detection

DOI Link

https://doi.org/10.13208/j.electrochem.140440

Publication Date

2015-02-28

Online Available Date

2014-10-16

Revised Date

2014-10-11

Received Date

2014-07-23

Recommended Citation

Jun-tao LIU, Yan-ling LIU, Zhi CHENG, Shi-jing CHEN, Wei-Hua HUANG. Electrochemical Monitoring of Cell Wall-Regulated Transient Extracellular Oxidative Burst from Single Plant Cells[J]. Journal of Electrochemistry, 2015 , 21(1): 29-38.
DOI: 10.13208/j.electrochem.140440
Available at: https://jelectrochem.xmu.edu.cn/journal/vol21/iss1/5