Abstract
We have developed a multi-channel electrochemical immunosensing system for the detection of α-fetoprotein (AFP). The sensing system consisted of carbon screen-printed electrode (SPCE) arrays and polyHRP-based signal amplification probes. AFP antigens could bind to both capture antibodies immobilized on electrode arrays ant detection antibodies (anti-AFP IgG developed in rabbit). The anti-rabbit IgG conjugated to poly-horseradish peroxidase were used as a signaling probe to bind to the immuno-complex. The current signals were harvested by a home-made multi-channels electrochemical detector. AFP could be detected in the concentration ranges of 64 pg·mL-1 ~ 250 ng·mL-1. The detection limit was as low as 56 pg·mL-1. The proposed immunosensing system provided a high sensitive, specific, simple and practical method for AFP detection.
Graphical Abstract
Keywords
multi-channel carbon screen-printed electrode, electrochemical immunosensing system, α-fetoprotein
Publication Date
2015-02-28
Online Available Date
2014-11-15
Revised Date
2014-11-09
Received Date
2014-09-10
Recommended Citation
Wang-ping DENG, Yan-zhi DOU, Jing SU, Lin HAO, Shi-ping SONG, Chun-hai FAN.
A Multi-channel Electrochemical Immunosensing System with PolyHRP-based Signal Amplification for the Detection of Tumor Markers[J]. Journal of Electrochemistry,
2015
,
21(1): 39-44.
DOI: 10.13208/j.electrochem.140443
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol21/iss1/6
References
[1] Yang L, Parkin D M, Ferlay J, et al. Estimates of cancer incidence in China for 2000 and projections for 2005[J]. Cancer Epidemiology Biomarkers & Prevention, 2005, 14(1): 243-250.
[2] Lin J H, Ju H X. Electrochemical and chemiluminescent immunosensors for tumor markers[J]. Biosensors and Bioelectronics, 2005, 20(8): 1461-1470.
[3] Hayes D F, Bast R C, Desch C E, et al. Tumor marker utility grading system: A framework to evaluate clinical utility of tumor markers[J]. Journal of the National Cancer Institute, 1996, 88(20): 1456-1466.
[4] Du D, Zou Z X, Yongsoon S, et al. Sensitive immunosensor for cancer biomarker based on dual signal amplification strategy of graphene sheets and multienzyme functionalized carbon nanospheres[J]. Analytical Chemistry, 2010, 82(7): 2989-2995.
[5] Wang G L, Xu J J, Chen H Y, et al. Label-free photoelectrochemical immunoassay for α-fetoprotein detection based on TiO2/CdS hybrid[J]. Biosensors and Bioelectronics, 2009, 25: 791-796.
[6] Zhao L F, Li S G, He J, et al. Enzyme-free electrochemical immunosensor con?gured with Au-Pd nanocrystals and N-doped graphene sheets for sensitive detection of AFP[J]. Biosensors and Bioelectronics, 2013, 49: 222-225.
[7] Zhou H K, Gan N, Li T H, et al. The sandwich-type electrochemiluminescence immunosensor for α-fetoprotein based on enrichment by Fe3O4-Au magnetic nano probes and signal ampli?cationby CdS-Au composite nanoparticles labeled anti-AFP[J]. Analytica Chimica Acta, 2012, 746: 107-113.
[8] Liang G D, Liu S F, Zou G Z. Ultrasensitive immunoassay based on anodic near-infrared electrochemiluminescence from dual-stabilizer-capped CdTe nanocrystals[J]. Analytical Chemistry, 2012, 84(24): 10654-10659.
[9] Hu M, Yan J, He Y, et al. Ultrasensitive, multiplexed detection of cancer biomarkers directly in serum by using a quantum dot-based microfluidic protein chip[J]. ACS Nano, 2009, 4(1): 488-494.
[10] Yang Z J, Liu H, Zong C. Automated support-resolution strategy for a one-way chemiluminescent multiplex immunoassay[J]. Analytical Chemistry, 2009, 81(13): 5484-5489.
[11] Wilson M S. Electrochemical immunosensors for the simultaneous detection of two tumor markers[J]. Analytical Chemistry, 2005, 77(5): 1496-1502.
[12] Jeonga Y, Choib K, Kimc J, et al. PDMS micro bead cage reactor for the detection of alpha fetoprotein (AFP)[J]. Sensors and Actuators B, 2008, 128(2): 349-358.
