A Multi-channel Electrochemical Immunosensing System with PolyHRP-based Signal Amplification for the Detection of Tumor Markers

Authors

Wang-ping DENG, Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;
Yan-zhi DOU, Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;
Jing SU, Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;
Lin HAO, Xuzhou Clinical Institute, Xuzhou Medical College, Xuzhou 221009, Jiangsu, China;
Shi-ping SONG, Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;Follow
Chun-hai FAN, Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;

Corresponding Author

Shi-ping SONG(songshiping@sinap.ac.cn)

Abstract

We have developed a multi-channel electrochemical immunosensing system for the detection of α-fetoprotein (AFP). The sensing system consisted of carbon screen-printed electrode (SPCE) arrays and polyHRP-based signal amplification probes. AFP antigens could bind to both capture antibodies immobilized on electrode arrays ant detection antibodies (anti-AFP IgG developed in rabbit). The anti-rabbit IgG conjugated to poly-horseradish peroxidase were used as a signaling probe to bind to the immuno-complex. The current signals were harvested by a home-made multi-channels electrochemical detector. AFP could be detected in the concentration ranges of 64 pg·mL^-1 ~ 250 ng·mL^-1. The detection limit was as low as 56 pg·mL^-1. The proposed immunosensing system provided a high sensitive, specific, simple and practical method for AFP detection.

Graphical Abstract

Keywords

multi-channel carbon screen-printed electrode, electrochemical immunosensing system, α-fetoprotein

DOI Link

https://doi.org/10.13208/j.electrochem.140443

Publication Date

2015-02-28

Online Available Date

2014-11-15

Revised Date

2014-11-09

Received Date

2014-09-10

Recommended Citation

Wang-ping DENG, Yan-zhi DOU, Jing SU, Lin HAO, Shi-ping SONG, Chun-hai FAN. A Multi-channel Electrochemical Immunosensing System with PolyHRP-based Signal Amplification for the Detection of Tumor Markers[J]. Journal of Electrochemistry, 2015 , 21(1): 39-44.
DOI: 10.13208/j.electrochem.140443
Available at: https://jelectrochem.xmu.edu.cn/journal/vol21/iss1/6