Corresponding Author

Xin-hua LIN(xinhua63@163.com)


A novel electrochemical platform for the high sensitivity detection of riboflavin was constructed by Au nanoparticles/polydopamine/carbon nanotubes (Au-PDA-MWCNTs) nanocomposite modified glassy carbon electrode. The Au-PDA-MWCNTs nanocomposite was synthesized by in situ reduction method. The characteristics of the as-prepared Au-PDA-MWCNTs nanocomposite modified electrodes were investigated by using UV-Vis spectroscopy, scanning electron microscopy (SEM) and electrochemical methods. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to study the electrochemical behavior of riboflavin (RF) at Au-PDA-MWCNTs nanocomposite modified electrodes. The results demonstrated that the present electrochemical sensor exhibited a wide linear range from 5×10-9 mol•L-1to 1×10-5 mol•L-1 for detection of riboflavin, with a detection limit of 1.7×10-9 mol•L-1 (S/N = 3). The present method for high sensitivity determination of riboflavin by electrochemical method at Au-PDA-MWCNTs nanocomposite modified electrodes is simple, accurate, reliable and feasible with an excellent anti-interference ability against electroactive species and metal ions. Accordingly, the present method proved to be useful for the estimation of the RF content in pharmaceutical samples with satisfactory recovery.

Graphical Abstract


riboflavin, gold nanoparticles, polydopamine, multi-walled carbon nanotubes, electrochemistry

Publication Date


Online Available Date


Revised Date


Received Date



[1] Chatterjee A, Foord J S. Biological applications of diamond electrodes: electrochemical studies of riboflavin[J]. Diamond and Related Materials, 2009, 18(5-8): 899-903.
[2] Massey V. The chemical and biological versatility of riboflavin[J], Biochemical Society Transactions, 2000, 28(4): 283-296.
[3] Sikorska E, Gliszczynska-Swig?o A, Insinska-Rak M, et al. Simultaneous analysis of riboflavin and aromatic amino acids in beer using fluorescence and multivariate calibration methods[J]. Analytica Chimica Acta, 2008, 613(2): 207-217.
[4] Qi H L, Cao Z Z, Hou L N. Electrogenerated chemiluminesence method for the determination of riboflavin at an ionic liquid modified gold electrode[J]. Spectrochimica Acta Part A,2011, 78, (1): 211-215.
[5] Lavanya N, Radhakrishnan S, Sekar C, et al. Fabrication of Cr doped SnO2 nanoparticles based biosensor for the selective determination of riboflavin in pharmaceuticals[J]. Analyst, 2013, 138(7): 2061-2067.
[6] Sá é S, Silva P S , Jost C L, et al. Electrochemical sensor based on bismuth-film electrode for voltammetric studies on vitamin B2 (riboflavin)[J]. Sensors and Actuators B: Chemical, 2015, 209(1): 423-430.
[7] Wang Y, Zhuang Q F, Ni Y N. Fabrication of riboflavin electrochemical sensor based on homoadenine single-stranded DNA/molybdenum disulfide€“graphene nanocomposite modified gold electrode[J]. Journal of Electroanalytical Chemistry, 2015, 736(1): 47-54.
[8] Zhang H, Zhao J S, Liu H T, et al. Application of poly (3-methylthiophene) modified glassy carbon electrode as riboflavin sensor[J]. International Journal of Electrochemical Science, 2010, 5: 295-301.
[9] Ahirwal G K, Mitra C K. Gold nanoparticles based sandwich electrochemical immunosensor[J]. Biosensors and Bioelectronics, 2010, 25(9): 2016-2020.
[10] Mani V, Dinesh B, Chen S M, et al. Direct electrochemistry of myoglobin at reduced graphene oxide-multiwalled carbon nanotubes-platinum nanoparticles nanocomposite and biosensing towards hydrogen peroxide and nitrite[J]. Biosensors and Bioelectronics, 2014, 53: 420-427.
[11] Lin L S, Cong Z X, Cao J B, et al. Fe3O4@polydopamine core-shell nanocomposites for intracellular mRNA detection and imaging-guided photothermal therapy[J]. ACS Nano, 2014, 8(4): 3876-3883.
[12] Loget G, Wood J B, Cho K, et al. Electrodeposition of polydopamine thin films for DNA patterning and microarrays[J]. Analytical Chemistry, 2013, 85(21): 9991-9995.
[13] Tsang S C, Chen Y K, Harris P J F, et al. A simple chemical method of opening and filling carbon nanotubes[J]. Nature, 1994, 372(6502): 159-162.
[14] Siebrands T, Giersig M, Mulvaney P, et al. Steric exclusion chromatography of nanometer-sized gold particles[J]. Langmuir, 1993, 9(9): 2297-2300.
[15] Zhang M, He X W, Chen L X, et al. Preparation of IDA-Cu functionalized core€“satellite Fe3O4/polydopamine/Au magnetic nanocomposites and their application for depletion of abundant protein in bovine blood[J]. Journal of Materials Chemistry, 2010, 47(20): 10696-10704.
[16] Wang T, Hu X G, Qu X H, et al. Noncovalent functionalization of multiwalled carbon nanotubes: Application in hybrid Nanostructures[ J]. Journal of Physical Chemistry B, 2006, 110(13): 6613-6636.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.