Corresponding Author

LIAO Ni(liaoni123456789@163.com)


In this paper, a novel electrochemiluminescence (ECL) immunosensor for the detection of apolipoprotein A1 was constructed based on flower-like platinum nanoparticles (PtNFs) via a one-pot chemical synthesis method. The PtNFs was used to immobilize the secondary antibody and enzyme (GOD). Then the prepared bioconjugates were introduced onto the electrode via sandwich immunoreactions. Accordingly, the ECL luminophore peroxydisulfate (S2O82- ) was presented in the working buffer solution containing an appropriate amount of glucose. Through the ECL responses of S2O82- and O2, a dramatically amplified ECL signal was obtained for the reason that hydrogen peroxide (H2O2) produced by GOD to glucose was subsequently catalyzed by PtNFs to in situ generate O2. The present immunosensor showed a wide linear range of 0.1 ng•mL-1 to 100 ng•mL-1, with a low detection limit of 0.03 ng•mL-1 for detecting Apo-A1. This new signal amplification strategy for preparation of ECL immunosensor could be easily realized and has potential application in ultrasensitive bioassays.

Graphical Abstract


flower-like structures platinum nanoparticles, electrochemiluminescence immunosensor, apolipoprotein A1

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