Direct Electrochemistry of Glucose Oxidase Based on WS₂ Quantum Dots and its Biosensing Application

Authors

Chen-lu LI, School of Chemistry and Material science, Key Lab for Functional Materials Chemistry of Guizhou Province, Guizhou normal university, Guiyang 550001，China;
Hua-ping PENG, Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350122，China;Follow
Zhong-nan HUANG, Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350122，China;
Yi-lun SHENG, Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350122，China;
Pei-wen WU, Department of Endocrinology, The Affiliated First Hospital, Fujian Medical University, Fuzhou 350005 , China;Follow
Xin-hua LIN, Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350122，China;

Corresponding Author

Hua-ping PENG(phpfjmu@126.com);
Pei-wen WU(247056892@qq.com)

Abstract

In this study, a novel electrochemical glucose biosensor has been developed by immobilizing glucose oxidase (GOx) on tungsten disulfide quantum dots (WS₂ QDs) on the surface of glassy carbon electrode (GCE). Transmission electron microscopy, UV-vis spectroscopy and cyclic voltammetry were employed to characterize the morphology, structure, and electrochemical behaviors of the as-prepared WS₂ QDs and the biofilm modified electrode. The results suggested that the WS₂ QDs could accelerate the electron transfer between the electrode and the immobilized enzyme, which enabled the direct electrochemistry of GOx without any electron mediator. Besides, the immobilized GOx in WS₂ QDsfilm exhibited excellent electrocatalytic activity toward oxidation of glucose due to the excellent biocompatibility of the WS₂ QDs. The proposed GOx/WS₂ QDs biofilm electrode exhibited a linear response to glucose concentration in the ranges of 25 ~ 100 μmol·L^-1 and 100 ~ 600 μmol·L^-1, and the detection limit of the biosensor was as low as 5.0 μmol·L^-1. The biosensor also exhibited good selectivity, reproducibility and long-term stability, which might be potenially used for the detection of the real samples.

Graphical Abstract

Keywords

tungsten disulfide quantum dots, glucose oxidase, direct electrochemistry, biosensor

DOI Link

https://doi.org/10.13208/j.electrochem.160829

Publication Date

2017-02-28

Online Available Date

2016-10-21

Revised Date

2016-09-23

Received Date

2016-08-29

Recommended Citation

Chen-lu LI, Hua-ping PENG, Zhong-nan HUANG, Yi-lun SHENG, Pei-wen WU, Xin-hua LIN. Direct Electrochemistry of Glucose Oxidase Based on WS₂ Quantum Dots and its Biosensing Application[J]. Journal of Electrochemistry, 2017 , 23(1): 53-58.
DOI: 10.13208/j.electrochem.160829
Available at: https://jelectrochem.xmu.edu.cn/journal/vol23/iss1/8