Corresponding Author

Hua-ping PENG(phpfjmu@126.com);
Pei-wen WU(247056892@qq.com)


In this study, a novel electrochemical glucose biosensor has been developed by immobilizing glucose oxidase (GOx) on tungsten disulfide quantum dots (WS2 QDs) on the surface of glassy carbon electrode (GCE). Transmission electron microscopy, UV-vis spectroscopy and cyclic voltammetry were employed to characterize the morphology, structure, and electrochemical behaviors of the as-prepared WS2 QDs and the biofilm modified electrode. The results suggested that the WS2 QDs could accelerate the electron transfer between the electrode and the immobilized enzyme, which enabled the direct electrochemistry of GOx without any electron mediator. Besides, the immobilized GOx in WS2 QDsfilm exhibited excellent electrocatalytic activity toward oxidation of glucose due to the excellent biocompatibility of the WS2 QDs. The proposed GOx/WS2 QDs biofilm electrode exhibited a linear response to glucose concentration in the ranges of 25 ~ 100 μmol·L-1 and 100 ~ 600 μmol·L-1, and the detection limit of the biosensor was as low as 5.0 μmol·L-1. The biosensor also exhibited good selectivity, reproducibility and long-term stability, which might be potenially used for the detection of the real samples.

Graphical Abstract


tungsten disulfide quantum dots, glucose oxidase, direct electrochemistry, biosensor

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