Sensitive and Reusable Electrogenerated Chemiluminescence Aptasensor Fabricated by Electrochemically Double Covalent Coupling Method for the Detection of Cocaine

Authors

Xiao-feia WANG, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, P.R. China;
Tinga ZHANG, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, P.R. China;
Bing WANG, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, P.R. China;
Hong-lan QI, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, P.R. China;Follow
Cheng-xiao ZHANG, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, P.R. China;Follow

Corresponding Author

Hong-lan QI(honglanqi@snnu.edu.cn);
Cheng-xiao ZHANG(cxzhang@snnu.edu.cn)

Abstract

A double covalent coupling method based on click chemistry and diazonium chemistry was developed for the fabrication of a sensitive and reusable electrogenerated chemiluminescence (ECL) aptasensor. As a proof-of-concept, cocaine was chosen as a model target, and a specific aptamer was chosen as a recognition molecule element, while ruthenium complex derivative (Ru1) as an ECL signal compound. The ECL aptasensor was fabricated by electrochemically diazotizing a phenylazide on the surface of a glassy carbon electrode, and then, coupling the alkyne functionalized Ru1-labeled cocaine aptamer through click chemistry. The ECL aptasensor was facilely used to determine cocaine in the range from 0.1 nmol·L^-1 to 100 nmol·L^-1 with the detection limit as low as 60 pmol·L^-1 within 30 min via “signal on” mode. The advantage of the ECL aptasensor was further exemplified by good stability and reusability. This work demonstrates that the double covalent coupling method is a promising approach for the fabrication of the ECL biosensor.

Graphical Abstract

Keywords

electrogenerated chemiluminescence, aptasensor, cocaine, click chemistry, diazotization

DOI Link

https://doi.org/10.13208/j.electrochem.181044

Publication Date

2019-04-28

Online Available Date

2019-01-29

Revised Date

2019-01-15

Received Date

2018-12-04

Recommended Citation

Xiao-feia WANG, Tinga ZHANG, Bing WANG, Hong-lan QI, Cheng-xiao ZHANG. Sensitive and Reusable Electrogenerated Chemiluminescence Aptasensor Fabricated by Electrochemically Double Covalent Coupling Method for the Detection of Cocaine[J]. Journal of Electrochemistry, 2019 , 25(2): 223-231.
DOI: 10.13208/j.electrochem.181044
Available at: https://jelectrochem.xmu.edu.cn/journal/vol25/iss2/7