Sensitive Photoelectrochemical Assay of Nucleic Acids Based on Catalytic Hairpin Assembly and Ru(NH₃)₆³⁺

Authors

Ya-min FU, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, Hunan, China;
Xiao-xia YAN, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, Hunan, China;
Xiao-hua ZHANG, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, Hunan, China;Follow
Jin-hua CHEN, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, Hunan, China;Follow

Corresponding Author

Xiao-hua ZHANG(mickyxie@hnu.edu.cn);
Jin-hua CHEN(chenjinhua@hnu.edu.cn)

Abstract

A simple “signal-on” photoelectrochemical (PEC) sensing platform for sensitive assay of nucleic acids was developed by coupling catalytic hairpin assembly (CHA) signal amplification strategy with Ru(NH₃)₆³⁺. Herein, cadmium sulfide (CdS) was deposited on the TiO₂/indium tin oxide (ITO) electrode by a method of successive ionic layer adsorption and reaction (SILAR), serving as one kind of photoelectric material to broaden absorption range of TiO₂ and to improve the photoelectric conversion efficiency. Thereafter, the capture DNA (C-DNA) was immobilized on the CdS/TiO₂/ITO electrode. Simultaneously, Au-hairpin DNA probe 1 (Au-HP1) and hairpin DNA probe 2 (HP2) were able to hybridize to produce many Au-HP1:HP2 complexes under the existence of target DNA (T-DNA) based on CHA process. After that, C-DNA could capture Au-HP1:HP2 complex, leading to lots of double-stranded DNAs on the electrode to load numerous Ru(NH₃)₆³⁺, which resulted in a remarkable increase of photocurrent. As a result, a wide linear range (10 fmol·L^-1 to 1500 fmol·L^-1) and a low detection limit (6.19 fmol·L^-1) toward T-DNA were achieved. The developed method would have great potential applications in bioanalysis, screening of new drugs and early disease diagnosis.

Graphical Abstract

Keywords

photoelectrochemical biosensor, nucleic acid, catalytic hairpin assembly, [Ru(NH3)6]Cl3, CdS, TiO2

DOI Link

https://doi.org/10.13208/j.electrochem.181051

Publication Date

2019-04-28

Online Available Date

2019-02-01

Revised Date

2019-01-09

Received Date

2018-12-18

Recommended Citation

Ya-min FU, Xiao-xia YAN, Xiao-hua ZHANG, Jin-hua CHEN. Sensitive Photoelectrochemical Assay of Nucleic Acids Based on Catalytic Hairpin Assembly and Ru(NH₃)₆³⁺[J]. Journal of Electrochemistry, 2019 , 25(2): 232-243.
DOI: 10.13208/j.electrochem.181051
Available at: https://jelectrochem.xmu.edu.cn/journal/vol25/iss2/8