Electrochemical Sensor Based on Magnetic Electrode Modified with Magnetic Molecularly Imprinted Nanoparticles Immobilized Hemoglobin for Determination of Hydrogen Peroxide

Authors

Yang YUAN, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China;
Jia-xin WANG, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China;
Yu-hua CAO, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China;Follow

Corresponding Author

Yu-hua CAO(yuhuacao64@gmail.com )

Abstract

In this work, the surface-imprinted technique was used to prepare magnetic hemoglobin (Hb) imprinted nanoparticles, using Fe₃O₄@SiO₂ NPs as the carrier, Hb as the template molecule, and tetraethyl orthosilicate (TEOS) as the imprinted polymer monomer. The nanoparticles had a core-shell structure, with magnetic Fe₃O₄ NPs as the core and Hb imprinted polymers as the shell. Therefore, Hb could be concentrated and fixed on the surface of the magnetic imprinted nanoparticles (MMIPs NPs). Furthermore, MMIPs NPs were immobilized with chitosan (CS) on the surface of a magnetic electrode to constitute Hb enzyme-like biosensor to catalyze the reduction of hydrogen peroxide (H₂O₂). Compared with magnetic non-imprinted polymer nanoparticles (MNIPs NPs), the MMIPs NPs biosensor enhanced the response by 14.3%. Notably, an introduction of a magnetic field made the biosensor more sensitive owing to the paramagnetism of MNIPs NPs, Hb and O₂ molecules. The reduction current of H₂O₂ on Hb/MMIPs NPs modified magnetic glassy carbon electrode increased by 60.0%. Under the optimum condition, the linear detection range of H₂O₂ was 25 ~ 200 μmol·L^-1 with the detection limit of 3 μmol·L^-1 (S/N = 3), which showed that Hb enzyme-like biosensor had a good catalytic performance for H₂O₂.

Graphical Abstract

Keywords

hemoglobin, enzyme-like biosensor, hydrogen peroxide, magnetic imprinted nanoparticles, magnetic electrode

DOI Link

https://doi.org/10.13208/j.electrochem.180704

Publication Date

2019-12-28

Online Available Date

2018-09-29

Revised Date

2018-08-24

Received Date

2018-07-04

Recommended Citation

Yang YUAN, Jia-xin WANG, Yu-hua CAO. Electrochemical Sensor Based on Magnetic Electrode Modified with Magnetic Molecularly Imprinted Nanoparticles Immobilized Hemoglobin for Determination of Hydrogen Peroxide[J]. Journal of Electrochemistry, 2019 , 25(6): 757-763.
DOI: 10.13208/j.electrochem.180704
Available at: https://jelectrochem.xmu.edu.cn/journal/vol25/iss6/13