Corresponding Author

Zuo-peng LI(lizuopeng@126.com);
Feng FENG(feng-feng64@263.net)


In this study, differential pulse stripping voltammetry was developed for the quantitative detection of levofloxacin hydrochloride indirectly. Levofloxacin hydrochloride contains organic amine, which can be precipitated upon interaction with Zn(SCN)42-. By means of differential pulse stripping voltammetry, the concentration of Zn2+ can be determined using an in-situ formed bismuth film electrode, which allows determining indirectly the amount of levofloxacine hydrochloride. The linearity equation, I = 1.1401c - 0.5309 with the correlative coefficient R = 0.9979, was presented in the experiment. The results showed that this approach is very sensitive, having a limitation of detection of 3.18 × 10-5 μg·mL-1 in a linearity range of 5.0 ~ 60 μg·mL-1.

Graphical Abstract


bismuth film electrode, differential pulse stripping voltammetry, levofloxacine hydrochloride, zinc ion, ion-association compound

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[1] Seifrtova M, Novakova L, Lino C, et al. An overview of analytical methodologies for the determination of antibiotics in environmental waters[J]. Analytica Chimica Acta, 2009, 649: 158-179.
[2] Wong F, Juzwin S, Flor S. Rapid stereo specific high-performance liquid chromatographic determination of levofloxacin in human plasma and urine[J]. Journal of Pharmaceutical and Biomedical Analysis, 1997, 15(6): 765-771.
[3] Bǒttcher S, Baum H, Hoppe-Tichy T, et al. An HPLC assay and a microbiological assay to determine levofloxacin in soft tissue, bone, bile and serum[J]. Journal of Pharmaceutical and Biomedical Analysis, 2001, 25(2): 197-203.
[4] Neckel U, Joukhadar C, Frossard M, et al. Simultaneous determination of levofloxacin and ciprofloxacin in microdialysates and plasma by high-performance liquid chromatography[J]. Analytica Chimica Acta, 2002, 463: 199-206.
[5] Liang H, Kays M B and Sowinski K M. Separation of levofloxacin, ciprofloxacin, gatifloxacin, moxifloxacin, trovafloxacin and cinoxacin by high-performance liquid chromatography: application to levofloxacin determination in human plasma[J]. Journal of Chromatography B, 2002, 772(1): 53-63.
[6] Hurtado F K, Laureano J V, Lock G, et al. Enhanced penetration of moxifloxacin into rat prostate tissue evidenced by microdialysis[J]. International Journal of Antimicrobial Agents, 2014, 44(4): 327-333.
[7] Cheng F C, Tsai T R, Chen Y F, et al. Pharmacokinetic study of levofloxacin in rat blood and bile by microdialysis and high-performance liquid chromatography[J]. Journal of Chromatography A, 2002, 961(1): 131-136.
[8] Gong Q J, Qiao J L, Du L M, et al. Recognition and simultaneous determination of levofloxacin enantiomers by synchronization-1st derivative fluorescence spectroscopy[J]. Talanta, 2000, 53(2): 359-365.
[9] González J A, Mochón M C, Rosa F C. Spectrofluorimetric determination of levofloxacin in tablets, human urine and serum[J]. Talanta, 2000, 52(6): 1149-1156.
[10] Herrera-Herreraa A V, Ravelo-Péreza L M, Hernández-Borgesa J, et al. Oxidized multi- walled carbon nanotubes for the dispersive solid-phase extraction of quinolone antibiotics from water samples using capillary electrophoresis and large volume sample stacking with polarity switching[J]. Journal of Chromatography A, 2011, 1218(31): 5352-5361.
[11] Huet A C, Charlier C, Tittlemier S A, et al. Simultaneous determination of (fluoro)quinolone antibiotics in kidney, marine products, eggs, and muscle by enzyme-linked immunosorbent assay (ELISA)[J]. Journal of Agricultural and Food Chemistry, 2006, 54(8): 2822-2827.
[12] Mazzotta E, Malitesta C, Díaz-álvarez M, et al. Electrosynthesis of molecularly imprinted polypyrrole for the antibiotic levofloxacin[J]. Thin Solid Films, 2012, 520(6): 1938-1943.
[13] Ghoneim M M, Radi A, Beltagi A M. Determination of norfloxacin by squarewave adsorptive voltammetry on a glassy carbon electrode[J]. Journal of Pharmaceutical and Biomedical Analysis, 2001, 25(2): 205-210.
[14] Radi A, Ries M A, Kandil S. Electrochemical study of the interaction of levofloxacin with DNA[J]. Analytica Chimica Acta, 2003, 495(1/2): 61-67.
[15] Radi A, El-Sherif Z. Determination of levofloxacin in human urine by adsorptive square-wave anodic stripping voltammetry on a glassy carbon electrode[J]. Talanta, 2002, 58(2): 319-324.
[16] Wen W, Zhao D M, Zhang X H, et al. One-step fabrication of poly(o-aminophenol)/multi-walled carbon nanotubes composite film modified electrode and its application for levofloxacin determination in pharmaceuticals[J]. Sensors and Actuators B: Chemical, 2012, 174: 202-209.
[17] Cesarino V, Cesarino I, Moraes F C, et al. Carbon nano-tubes modified with SnO2 rods for levofloxacin detection[J]. Journal of the Brazilian Chemical Society, 2014, 25: 502-508.
[18] Tang T, Tong Y, Zheng R F, et al. Ag nanoparticles and electrospun CeO2-Au composite nanofibers modified glassy carbon electrode for determination of levofloxacin[J]. Sensors and Actuators B: Chemical, 2014, 203: 95-101.
[19] Rutyna I, Korolczuk M. Determination of lead and cadmium by anodic stripping voltammetry at bismuth film electrodes following double deposition and stripping steps[J]. Sensors and Actuators B: Chemical, 2014, 204: 136-141.
[20] Santos V B, Fava E L, Curi N S, et al. A thermostated electrochemical flow cell with acoupled bismuth film electrode for square-wave anodic stripping voltammetric determination of cadmium(II) and lead(II) innatural, waste water and tap water samples[J]. Talanta, 2014, 126: 82-90.
[21] Sopha H, Hocevar S B, Pihlar B, et al. Bismuth film electrode for stripping voltammetric measurement of sildenafil citrate[J]. Electrochimica Acta, 2012, 60: 274-277.
[22] Gerent G G, Gonçalves C Q, Silva P S, et al. In situ bismuth film electrode for square wave cathodic voltammetric detection of pendimethalin at nanomolar level[J]. Electrochimica Acta, 2015, 168: 379-385.
[23] Romiha T, Hoěvarb S B, Jemeca A, et al. Bismuth film electrode for anodic stripping voltammetric measurement of silver nanoparticle dissolution[J]. Electrochimica Acta, 2016, 188: 393-397.
[24] Skoog D A. Fundamentals of Analytical Chemistry(9th edition)[M]. Belment USA: Thomson Brooks/Cole, 2014.
[25] Sarkar D, Pramanik A, Jana S, et al. Quinoline based reversible fluorescent €˜turn-on€™ chemosensor for the selective detection of Zn2+: Application in living cell imaging and as INHIBIT logic gate[J]. Sensors and Actuators B: Chemical, 2015, 209: 138-146.
[26] Sultana N, Arayne M S, Rizvi S B S, et al. Synthesis, spectroscopic, and biological evaluation of some levofloxacin metal complexes[J]. Medicinal Chemistry Research, 2013, 22(3): 1371-1377.
[27] Sadeek S A, EI-Shwiniy W H. Preparation, structural characterization and biological studies of some new levofloxacin metal complexes[J]. Journal of the Iranian Chemical Society, 2017, 14(8): 1711-1723.
[28] Refat M S. Synthesis and characterization of norfloxacin-transition metal complexes (group 11, IB): Spectroscopic, thermal, kinetic measurements and biological activity[J]. Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy 2007, 68(5): 1393-1045.
[29] Majumdar D, Babu M S S, Das S, et al. Syntheses, X-ray crystal structures, photoluminescence properties, antimicrobial activities and hirshfeld surface of two new Cd(II) azide/thiocyanate linked coordination polymers[J]. ChemistrySelect, 2017, 2(17): 4811-4822.



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