An Aptasensor Based on AuNPs/PANI/TNTs Nanocomposite for Electrochemical Detection of Tobramycin

Authors

Yong-ling NONG, School of Chemistry and Chemical Engineering, GuangDong Pharmaceutical University, Guangzhou, 510006, China;
Ni-na QIAO, School of Chemistry and Chemical Engineering, GuangDong Pharmaceutical University, Guangzhou, 510006, China;
Ying LIANG, School of Chemistry and Chemical Engineering, GuangDong Pharmaceutical University, Guangzhou, 510006, China;Guangdong Cosmetics Engineering & Technology Research Center, Guangzhou, 510006, China;Follow

Corresponding Author

Ying LIANG(lyingair@126.com)

Abstract

A novel well-constructed electrochemical aptamer-based sensor for the detection of tobramycin was presented, using differential pulse voltammetry (DPV) as a detection technique and methylene blue（MB） as an electrochemical indicator. A glassy carbon electrode modified with a nanocomposite of Au nanoparticles/polyaniline/titania nanotubes (AuNPs/PANI/TNTs) was constructed as the electrode scaffold. The nanocomposite was characterized by transmission electron microscopy and X-ray photoelectron spectroscopy in detail. The results of cyclic voltammetry and electrochemical impedance measurements demonstrated that the AuNPs/PANI/TNTs nanocomposites can improve greatly the electron transfer on the interface. For the detection of tobramycin, the DPV results showed a linear relationship between the current response and the concentration of tobramycin, and a wide range of detection from 0.5 μmol·L^-1 to 70 μmol·L^-1. The presented aptamer-based biosensor exhibited excellent sensitivity and reproducibility, which would have a potential application in bioanalysis and clinical diagnostics.

Graphical Abstract

Keywords

aptasensor, tobramycin, electrochemical analysis, Au nanoparticles

DOI Link

https://doi.org/10.13208/j.electrochem.180612

Publication Date

2019-12-28

Online Available Date

2018-08-10

Revised Date

2018-07-14

Received Date

2018-06-12

Recommended Citation

Yong-ling NONG, Ni-na QIAO, Ying LIANG. An Aptasensor Based on AuNPs/PANI/TNTs Nanocomposite for Electrochemical Detection of Tobramycin[J]. Journal of Electrochemistry, 2019 , 25(6): 720-730.
DOI: 10.13208/j.electrochem.180612
Available at: https://jelectrochem.xmu.edu.cn/journal/vol25/iss6/9