Abstract
Herein, sulfamic acid (SA) was utilized, for the first time, to enhance significantly the luminol electrochemiluminescence (ECL). With the SA concentration increased from 0.1 μmol·L-1 to 500 μmol·L-1 the ECL intensity increased proportionally. The developed luminol/SA ECL system was employed to detect dopamine (DA) based on its prominent quenching effect. The Stern-Volmer equation of Io/I= 1+Ksv[DA] could be applied to express well the quenching mechanism of DA in the luminol/SA ECL system. The calibration plot showed that the increase in the DA concentration from 0.5 to 20 μmol·L-1 decreased linearly the ECL intensity with a detection limit of 30 nmol·L-1. The luminol/SA ECL system was successfully carried out for DA detection in urine real sample by employing the standard addition method with the excellent recoveries of 103% ~ 105%. Selectivity of the as-developed ECL system was also investigated by using uric acid, ascorbic acid, sugars and amino acids. The results indicated that the ECL intensities changed negligibly in the presence of other substances.
Graphical Abstract
Keywords
electrochemiluminescence, sulfamic acid, luminol, dopamine
Publication Date
2021-04-28
Online Available Date
2021-03-15
Revised Date
2021-03-08
Received Date
2021-02-02
Recommended Citation
Hailemariam Barkae Tesfaye, Ibrahim Halawa Mohamed, Haile Fereja Tadesse, Addisu Kitte Shimeles, Ma Xian-Gui, Chen Ye-Quan, Xu Guo-Bao.
Luminol/Sulfamic Acid Electrochemiluminescence and Its Application for Dopamine Detection[J]. Journal of Electrochemistry,
2021
,
27(2): 168-176.
DOI: Herein, sulfamic acid (SA) was utilized, for the first time, to enhance significantly the luminol electrochemiluminescence (ECL). With the SA concentration increased from 0.1 μmol·L-1 to 500 μmol·L-1 the ECL intensity increased proportionally. The developed luminol/SA ECL system was employed to detect dopamine (DA) based on its prominent quenching effect. The Stern-Volmer equation of Io/I= 1+Ksv[DA] could be applied to express well the quenching mechanism of DA in the luminol/SA ECL system. The calibration plot showed that the increase in the DA concentration from 0.5 to 20 μmol·L-1 decreased linearly the ECL intensity with a detection limit of 30 nmol·L-1. The luminol/SA ECL system was successfully carried out for DA detection in urine real sample by employing the standard addition method with the excellent recoveries of 103% ~ 105%. Selectivity of the as-developed ECL system was also investigated by using uric acid, ascorbic acid, sugars and amino acids. The results indicated that the ECL intensities changed negligibly in the presence of other substances.
Available at: https://jelectrochem.xmu.edu.cn/journal/vol27/iss2/5