Luminol/Sulfamic Acid Electrochemiluminescence and Its Application for Dopamine Detection

Hailemariam Barkae Tesfaye, 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin,China;2. School of Applied chemistry and Engineering, University of Science and Technology of China, Hefei 230026, Anhui, China;3. Wolkite university, College of Natural & Computational Science, Department of Chemistry, P.O Box 07, Wolkite, Ethiopia;
Ibrahim Halawa Mohamed, 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin,China;4. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, 35516, Mansoura, Egypt;5. College of Chemistry and Environmental Engineering and College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, Guangdong, China;
Haile Fereja Tadesse, 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin,China;6. Ambo University, College of Medicine and Health Sciences, Department of Pharmacy, P. O. Box 19, Ambo, Ethiopia;
Addisu Kitte Shimeles, 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin,China;7. Jimma University, College of Natural Sciences, Department of Chemistry, P. O. Box 378, Jimma, Ethiopia;
Xian-Gui Ma, 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin,China;2. School of Applied chemistry and Engineering, University of Science and Technology of China, Hefei 230026, Anhui, China;
Ye-Quan Chen, 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin,China;
Guo-Bao Xu, 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin,China;Follow

Corresponding Author

Xu Guo-Bao(guobaoxu@ciac.ac.cn)

Abstract

Herein, sulfamic acid (SA) was utilized, for the first time, to enhance significantly the luminol electrochemiluminescence (ECL). With the SA concentration increased from 0.1 μmol·L^-1 to 500 μmol·L^-1 the ECL intensity increased proportionally. The developed luminol/SA ECL system was employed to detect dopamine (DA) based on its prominent quenching effect. The Stern-Volmer equation of I_o/I= 1+K_sv[DA] could be applied to express well the quenching mechanism of DA in the luminol/SA ECL system. The calibration plot showed that the increase in the DA concentration from 0.5 to 20 μmol·L^-1 decreased linearly the ECL intensity with a detection limit of 30 nmol·L^-1. The luminol/SA ECL system was successfully carried out for DA detection in urine real sample by employing the standard addition method with the excellent recoveries of 103% ~ 105%. Selectivity of the as-developed ECL system was also investigated by using uric acid, ascorbic acid, sugars and amino acids. The results indicated that the ECL intensities changed negligibly in the presence of other substances.

Graphical Abstract

Keywords

electrochemiluminescence, sulfamic acid, luminol, dopamine

DOI Link

https://doi.org/10.13208/j.electrochem.201247

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Publication Date

2021-04-28

Online Available Date

2021-03-15

Revised Date

2021-03-08

Received Date

2021-02-02

Recommended Citation

Hailemariam Barkae Tesfaye, Ibrahim Halawa Mohamed, Haile Fereja Tadesse, Addisu Kitte Shimeles, Xian-Gui Ma, Ye-Quan Chen, Guo-Bao Xu. Luminol/Sulfamic Acid Electrochemiluminescence and Its Application for Dopamine Detection[J]. Journal of Electrochemistry, 2021 , 27(2): 168-176.
DOI: Herein, sulfamic acid (SA) was utilized, for the first time, to enhance significantly the luminol electrochemiluminescence (ECL). With the SA concentration increased from 0.1 μmol·L^-1 to 500 μmol·L^-1 the ECL intensity increased proportionally. The developed luminol/SA ECL system was employed to detect dopamine (DA) based on its prominent quenching effect. The Stern-Volmer equation of I_o/I= 1+K_sv[DA] could be applied to express well the quenching mechanism of DA in the luminol/SA ECL system. The calibration plot showed that the increase in the DA concentration from 0.5 to 20 μmol·L^-1 decreased linearly the ECL intensity with a detection limit of 30 nmol·L^-1. The luminol/SA ECL system was successfully carried out for DA detection in urine real sample by employing the standard addition method with the excellent recoveries of 103% ~ 105%. Selectivity of the as-developed ECL system was also investigated by using uric acid, ascorbic acid, sugars and amino acids. The results indicated that the ECL intensities changed negligibly in the presence of other substances.