Abstract
The interaction between rare earth ions(e.g. Eu3+ ,Ce3+, Er3+, Sm3+, Tb3+, La3+ and Nd3+) and DNA had been investigated by using Cyclic Voltammetry, Ultraviolet and Raman Spectroscopy. After the adding of Ce3+ ion to DNA solution, the cathodic peak current of Ce3+ ion reduction was found to decrease obviously, and the anodic peak current to decrease even more significantly. Differential UV spectra of Ce3+ ion depicted hyperchromic effect after the reaction of these two species and a new peak of 272 nm was found. Differential UV spectra of DNA also showed the hyperchromic effect. The peak of 814 cm-1 disappeared in the Raman spectra of DNA. All of above results may be interpreted as the cleavage effect of cerium ion on DNA. Similar effect were observed between Eu3+ ion and DNA, but less significant than those observed in Ce3+-DNA system. Probably due to the electrostatic interaction between Eu3+ and DNA and the stack effect of the base in DNA. Peak current decreasing and hyperchromic effect were also observed in cases of reaction of DNA with Er3+, Sm3+, Tb3+, La3+ and Nd3+ , but all weaker than in Cu3+-DNA system. The cleavage effect of Ce3+ on the phosphodiest linkage in DNA may be useful in employing the Ce3+ ion as the “Molecular scission” for catalytically hydrolytic cleavage of DNA.
Keywords
DNA, Rare earth ions, Cyclic voltammetry, UV and Raman spectroscopy
Publication Date
1998-11-28
Online Available Date
1998-11-28
Revised Date
1998-04-21
Received Date
1998-01-20
Recommended Citation
Hei-bai SHEN, Yu-zhuan KANG, Hai-feng YANG, Lin YU, Zong-rang ZHANG.
Study of the Effect of Rare Earth Ions on DNA Using Cyclic Voltammetry and Spectroscopy[J]. Journal of Electrochemistry,
1998
,
4(4): 400-405.
DOI: 10.61558/2993-074X.3147
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol4/iss4/12
Included in
Analytical Chemistry Commons, Materials Chemistry Commons, Nanoscience and Nanotechnology Commons, Physical Chemistry Commons
