Immobilization of Long-Strand DNA onto Gold Surface and Electrochemical Labeling of Hybridization of DNA

Authors

Jian zhang ZHOU, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China;
Ling ling WU, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China;
Li qin DONG, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China;
Zhong-yu LIN, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China;
Jia wei YAN, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China;
Ping DONG, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China;
Yue BAO, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China;
Zhong hua LIN, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China;

Abstract

Poly(diallyldimethylammonium chloride)(PDDA) was used to facilitate long strand DNA immobilization onto gold surface using self assembly method. Characterization of the PDDA self assembled film and subsequently immobilized DNA were carried out by Diffuse Reflectance Infrared, XPS, STM and electrochemical measurements. Hybridization of DNA was labeled by electrochemical method.

Keywords

Polyelectrolyte, Immobilization, Electrochemical labeling, STM, FT IR, XPS

DOI Link

https://doi.org/10.61558/2993-074X.1426

Publication Date

2001-08-28

Online Available Date

2001-08-28

Revised Date

2001-08-28

Received Date

2001-08-28

Recommended Citation

Jian zhang ZHOU, Ling ling WU, Li qin DONG, Zhong-yu LIN, Jia wei YAN, Ping DONG, Yue BAO, Zhong hua LIN. Immobilization of Long-Strand DNA onto Gold Surface and Electrochemical Labeling of Hybridization of DNA[J]. Journal of Electrochemistry, 2001 , 7(3): 276-280.
DOI: 10.61558/2993-074X.1426
Available at: https://jelectrochem.xmu.edu.cn/journal/vol7/iss3/2

References

[1]　BonchevaM ,SchiblerL ,LincolnP ,etal.Designofoligonucleotidearraysatinterfaces[J].Langmuir,1999,15:4317.

[2]　ThompsonM ,FurtadoLM .HighdensityoligonucleotideDNAprobearraysfortheanalysisattargetDNA[J].Analyst,1999,124:1133.

[3]　TatonTA ,MirkinCA ,LetsingerRL .ScanometricDNAarraydetectionwithnanoparticleprobes[J].Sci ence,2000,289:1757.

[4]　ReichertJ,Cs偄kiA,K hlerJM ,etal.Chip_basedopticaldetectionatDNAhybridizationbymeansofnanobeadlabeling[J].Anal.Chem.,2000,72:6025.

[5]　LipshuzeRJ,FodorSPA ,etal.Highdensitysyntheticoligonucleotidearrays[J].NatureGenetics,1999,21(1):120.

[6]　CheungVG ,MorleyM ,etal.Makingandreadingmicroarrays[J].NatureGenetics,1999,21(1):115.

[7]　WuLL ,ZhouJZ .XUJ ,etal.ElectrochemicalLabelingofNucleicAcid,ExtendedAbstractsof10 thNa tionalCongressofElectrochemistryofCHINA[C],HangZhou,1999,D004.

[8]　RoeDK ,GerischerH .Preparationofsphericalsinglecrystalelectrodesforuseinelectrocrystallizationstudies[J].J .Electrochem.Soc.,1963,110:350.

[9]　TsuboiM .InfraredandRamanSpectroscopy:BasicPriciplesinNucleicAcidChemistryVol.1[M ].NewYorkandLondon:AcademicPress,1974.

[10]　WuLL ,ZhouJZ ,LuoJ ,etal.Oxidationandadsorptionofdeoxybonucleicacidathighlyorderedpyrolyticgraphiteelectrode[J].ElectrochimicaActa,2000,45:2923.

[11]　PangDW ,AbrunaHD .MicromethodfortheinvestigationoftheinteractionsbetweenDNAandredox_ac tivemolecules[J].Anal.Chem.,1998,70:3162