Immobilization of Long-Strand DNA onto Gold Surface and Electrochemical Labeling of Hybridization of DNA

Authors

Jian-zhang ZHOU, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China
Ling-ling WU, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China
Li-qin DONG, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China
Zhong-yu LIN, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China
Jia-wei YAN, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China
Ping DONG, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China
Yue BAO, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China
Zhong-hua LIN, State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, ChinaFollow

Corresponding Author

Zhong-hua LIN(zhlin@xmu.edu.cn)

Abstract

Poly(diallyldimethylammonium chloride)(PDDA) was used to facilitate long strand DNA immobilization onto gold surface using self assembly method. Characterization of the PDDA self assembled film and subsequently immobilized DNA were carried out by Diffuse Reflectance Infrared, XPS, STM and electrochemical measurements. Hybridization of DNA was labeled by electrochemical method.

Keywords

Polyelectrolyte, Immobilization, Electrochemical labeling, STM, FT-IR, XPS

DOI Link

https://doi.org/10.61558/2993-074X.1426

Publication Date

2001-08-28

Online Available Date

2001-08-28

Revised Date

2001-08-20

Received Date

2001-03-10

Recommended Citation

Jian-zhang ZHOU, Ling-ling WU, Li-qin DONG, Zhong-yu LIN, Jia-wei YAN, Ping DONG, Yue BAO, Zhong-hua LIN. Immobilization of Long-Strand DNA onto Gold Surface and Electrochemical Labeling of Hybridization of DNA[J]. Journal of Electrochemistry, 2001 , 7(3): 276-280.
DOI: 10.61558/2993-074X.1426
Available at: https://jelectrochem.xmu.edu.cn/journal/vol7/iss3/2