Electrochemical Properties of Nitrate Immobilized on Gold Electrodes with 2-aminoethanethiol SAMs

Authors

Ya-ru SONG, Department of Chemistry, Beijing Normal University, Beijing 100875, China
Hong WU, Department of Chemistry, Beijing Normal University, Beijing 100875, China
Hui-bo SHAO, Department of Chemistry, Beijing Normal University, Beijing 100875, ChinaFollow

Corresponding Author

Hui-bo SHAO(hbs@bit.edu.cn)

Abstract

Nitrate reductase (NR) and phospholipids were immobilized on gold electrodes with 2-aminoethanethio(AET) SAMs. After Au/AET/NR and Au/AET/NR/lipid were soaked in pH 7 phosphate buffers for days, a pair of pre-reversible peak appeared at around 0.202 V and 0.149 V respsctively, irregularly grew with soaking time in blank buffer.The cathodic peak current (i_pcl) of Au/AET/NR system had linear relationship with scan rate between 0.02 and 0.5 V⋅s^-1 ,while i_pcl was proportional to V^1/2 in Au/AET/NR/lipid system. The direct electron transfer of enzyme electrodes was strongly affected by pH. Compared with Au/AET, NR exhibited a higher electrochemical activity in Au/AET/lipid system.

Keywords

Nitrate reductase, Phospholipid, 2-aminoethanethiol SAMs

DOI Link

https://doi.org/10.61558/2993-074X.3302

Publication Date

2002-08-28

Online Available Date

2002-08-28

Revised Date

2002-08-20

Received Date

2001-12-17

Recommended Citation

Ya-ru SONG, Hong WU, Hui-bo SHAO. Electrochemical Properties of Nitrate Immobilized on Gold Electrodes with 2-aminoethanethiol SAMs[J]. Journal of Electrochemistry, 2002 , 8(3): 333-336.
DOI: 10.61558/2993-074X.3302
Available at: https://jelectrochem.xmu.edu.cn/journal/vol8/iss3/17