Acetylcholinesterase Biosensor Platform Based on BP2000 for the Detection of Carbaryl

Authors

Ming-han ZHANG, College of Chemistry and Material Science, Jinan University, Guangzhou 511400, China;Jilin Laboratory of Advanced Power, Changchun Institute of Applied Chemistry,Changchun 130022, China;
Jun-qiao JIANG, School of Science, Changchun Institute of Technology, Changchun 130012, Jilin, China.;
Jun-jie Ge, Jilin Laboratory of Advanced Power, Changchun Institute of Applied Chemistry,Changchun 130022, China;
Wei XING, College of Chemistry and Material Science, Jinan University, Guangzhou 511400, China;Jilin Laboratory of Advanced Power, Changchun Institute of Applied Chemistry,Changchun 130022, China;Follow

Corresponding Author

Wei XING(zmzhang@jlu.edu.cn)

Abstract

With the purpose of providing a new method for carbaryl (a pesticide) detection, on the basis of the principle that acetylcholinesterase (AChE) activity can be restrained by carbaryl, an AChE biosensor platform based on BP2000 (as a fixation) was constructed by dropping method. As a result, it revealed that AChE immobilized on BP2000 maintained its catalytic activity for acetylcholine (ATCl), and due to the introduction of the BP2000 material, the effective electrochemical surface area of the modified electrode was enlarged. In addition, the electrochemical oxidation at the modified electrode occurred at low potential (0.630 V) accompanied by proton transmission. The AChE biosensor platform based on BP2000 matrix for carbaryl detection was able to reflect a linear response in the range of 2.0 ng·mL^-1 ~ 12.5 ng·mL^-1 with the detection limit of 3.15 ng·mL^-1. At last, this work will provide a simple method and an efficient matrix in establishing an enzyme electrode of enzymatic biosensor platform and enzymatic fuel cell.

Graphical Abstract

Keywords

acetylcholinesterase, carbaryl, enzyme-based biosensor, electrochemistry, black pearl 2000

DOI Link

https://doi.org/10.13208/j.electrochem.171229

Publication Date

2018-08-28

Online Available Date

2018-02-26

Revised Date

2018-01-30

Received Date

2017-12-29

Recommended Citation

Ming-han ZHANG, Jun-qiao JIANG, Jun-jie Ge, Wei XING. Acetylcholinesterase Biosensor Platform Based on BP2000 for the Detection of Carbaryl[J]. Journal of Electrochemistry, 2018 , 24(4): 303-308.
DOI: 10.13208/j.electrochem.171229
Available at: https://jelectrochem.xmu.edu.cn/journal/vol24/iss4/1