Abstract
Self-assembled monolayers (SAMs), which form highly ordered monolayers on the electrode surface through the gold-suffer bond, have attracted much attention in recent years. This stable layer not only can regulate the wettable properties of surface, but also can act as a promoter towards redox-active molecules. Here, we developed a simple and effective method to construct cysteine and cystamine co-self-assembled monolayer on gold microelectrode for in vivo detection of ascorbic acid (AA). The molar ratio at 1:1 of mixed monolayer has been found the optimum to enhance the electron-transfer kinetics of AA oxidation at low potential (ca. 0.10 V), meanwhile, it could resist the non-specific adsorption of protein at electrode surface. The application of the co-self-assembled monolayer is preliminarily demonstrated for in vivo detection and the basal level of striatum AA was determined to be 257 ± 30 mmol·L-1 (n = 3). This study offers a general and effective approach for in vivo electrochemistry with high reliability and simplified procedures.
Graphical Abstract
Keywords
cysteine and cystamine, co-self-assembled monolayer, ascorbic acid, anti-adsorption, in vivo
Publication Date
2019-06-25
Online Available Date
2019-03-19
Revised Date
2019-03-19
Received Date
2018-12-12
Recommended Citation
Yue ZHANG, Tao-tao FENG, Wen-liang JI, Mei-ning ZHANG.
Cysteine and Cystamine Co-Self-Assembled Monolayers for in Vivo Detection of Ascorbic Acid[J]. Journal of Electrochemistry,
2019
,
25(3): 400-408.
DOI: 10.13208/j.electrochem.181045
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol25/iss3/11
