A Low Noise Temperature Control System for Nanopore-Based Single Molecule Analysis

Authors

Cheng-yu YANG, 1. School of Chemistry & Molecular Engineering, East China University of Science and Technology,;
Zhen GU, 1. School of Chemistry & Molecular Engineering, East China University of Science and Technology,;
Zheng-li HU, 1. School of Chemistry & Molecular Engineering, East China University of Science and Technology,;
Yi-lun YING, 1. School of Chemistry & Molecular Engineering, East China University of Science and Technology,;Follow
Yi-tao LONG, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China; 2. Beijing National;

Corresponding Author

Yi-lun YING(yilunying@ecust.edu.cn)

Abstract

Nanopore employs a single bio-molecule interface, which is a highly sensitive single-molecule detection technology for measuring single biomolecules such as DNA, RNA, protein, and peptide. The interaction between single molecule and nanopore is thermodynamically controlled. Therefore, it is urgent to precisely control the temperature of the nanopore system without introduction of any noise. In this paper, we have developed a low-noise temperature control system for single-molecule detection of nanopores to achieve precise regulation at the ambient temperature during measurements. The system utilizes the thermoelectric effect of the semiconductor refrigerating chip to heat or cool the detection chamber, while adopts electromagnetically shielded high-precision thermistor to achieve the temperature feedback which avoids the introduction of noise at the same time. The proportional-integral-differential algorithm is used to provide the high-precision and rapid temperature control. The system was further applied in measuring the interaction between Poly(dA)5 and aerolysin nanopore at 25 ^oC to 5 ^oC. This system could be used to characterize the thermodynamic and kinetic information of the single molecules.

Graphical Abstract

Keywords

nanopore, electrochemistry, single bio-molecule detection, single bio-molecule interface, temperature control system, low noise measurement

DOI Link

https://doi.org/10.13208/j.electrochem.181056

Publication Date

2019-06-25

Online Available Date

2019-06-25

Revised Date

2019-03-20

Received Date

2019-01-02

Recommended Citation

Cheng-yu YANG, Zhen GU, Zheng-li HU, Yi-lun YING, Yi-tao LONG. A Low Noise Temperature Control System for Nanopore-Based Single Molecule Analysis[J]. Journal of Electrochemistry, 2019 , 25(3): 312-318.
DOI: 10.13208/j.electrochem.181056
Available at: https://jelectrochem.xmu.edu.cn/journal/vol25/iss3/3